MCF7-Luc
CBP30105L
| I. Introduction | |
| Host Cell: | MCF7 ER positive |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+ 10 µg/ml human insulin+ 1μg/ml Puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens,human |
| Tissue: | Mammary gland breast;derived from metastaticsite:pleural effusion |
| Disease: | Adenocarcinoma |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
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Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly recommend purchasing from Cobioer). |
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| IV. Stablity Test | |
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Figure 2. Stability test results. MCF7-Luc cells showed at least 32 passage stability with similar luminescence unit. |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
NOD SCID, 8weeks, Female |
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Implanted Position |
Intra-tibial injection |
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Injected Cell Numbers |
5×10^6/25 ul |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 3. MCF7-luc Mean Body Weight ± SEM.
2. Tumor Flux
Figure 4. Total flux MCF7-luc.
Orthotopic mammary fat pad：
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Index |
Content |
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Animal |
NOD SCID, 8weeks, Female |
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Implanted Position |
Orthotopic mammary fat pad |
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Injected Cell Numbers |
1×10^7/0.1 mL |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 5. MCF7-luc Mean Body Weight ± SEM.
2. Tumor Volume
Figure 6. MCF7-luc Mean Tumor Volume± SEM.
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
